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Human IFN-γ ELISA kitEK0458

  Catalog No. Package Size Price
EK0458-1 1x96T $399.00
EK0458-2 5x96T $1799.00
EK0458-3 10x96T $3459.00

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Description
  • Product Name Human IFN-γ ELISA kit
  • Brief Description ELISA Kit
  • Applications Solid Phase Sandwich ELISA
  • Species Reactivity Human
  • Specificity Natural and recombinant Human IFN-γ Ligand
  • Crossing Reactivity No significant interference observed with available related molecules.
  • Target Name Human IFN-γ
Application Details

Detect Range: 15.625-1000pg/ml
Sensitivity: 7pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hours
Detection method: Colorimetric

Images
  • Human IFN-γ ELISA kit - Absci

    Representative standard curve for IFN-γ ELISA. IFN-γ was diluted in serial two-fold steps in Sample Diluent.

Product Description
  • Aluminium pouches with a Microwell Plate coated with antibody to human IFN-γ (8 x 12)
  • 2 vials human IFN-γ Standard lyophilized, 4000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human IFN-γ antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
Background

Interferon gamma (IFN-γ) is a multifunctional protein first observed as an antiviral activity in cultures of Sindbis virus-infected human leukocytes stimulated by PHA (1). Produced by Tlymphocytes and natural killer (NK) cells, IFN-γ ?is now known to be both an inhibitor of viral replication and a regulator of numerous immunological functions. Human IFN-γ? is reported to be active only on human and non-human primate cells (5). The biochemistry and biological activities of the interferons have been extensively reviewed (2-9).

Human IFN-γ ?is a 143 amino acid residue, 20 or 25 kDa glycoprotein that demonstrates little sequence homology to IFN-α? or -β?(10-13). Naturally occurring IFN-γ ?is found as either of two molecular weight species, differing in degree of glycosylation. Human IFN-γ? apparently exists as a head-to-tail dimer in solution with the C-terminus of one monomer aligned with the N-terminus of the other monomer (14,15) A receptor for IFN-γ has been identified and its gene localized to chromosome 6 (16,17) Apparently the product of a single gene, the receptor is a single chain 90 kDa glycoprotein that shows a high degree of species-specific binding of IFN-γ?(18-21).

Functionally, IFN-γ ?produces a variety of effects. Produced by CD8+, NK, gd, and TH1 T helper cells, IFN-γ ?has documented antiviral, antiprotozoal and immunomodulatory effects on cell proliferation and apoptosis, as well as the stimulation and repression of a variety of genes (9, 22-25) he antiprotozoal activity of IFN-γ ?against Toxoplasma and Chlamydia is believed to result from indoleamine 2,3-dioxygenase activity, an enzyme induced by IFN-γ ?(26).The immunomodulatory effects of IFN-γ? are extensive and diverse. In monocyte/macrophages, the activities of IFN-γ ?include: increasing the expression of class I and II MHC antigens; increasing the production of IL-1, platelet-activating factor, H2O2, and pterin; protection of monocytes against LAK cell-mediated lysis; downregulation of IL-8 mRNA expression that is upregulated by IL-2; and, with lipopolysaccharide, induction of NO production.Finally, IFN-γ?has been shown to upregulate ICAM-1, but not E-Selectin or VCAM-1, expression on endothelial cells.

Regerences

Wheelock, E.F. (1965) Science 149:310.

Ijzermans, J.M. and R.L. Marquet (1989) Immunobiol. 179:456.

Mogensen, S.C. and J.L. Virelizier (1987) Interferon 8:55.

Grossberg, S.E. et al. (1989) Experientia 45:508.

Adolf, G.R. (1985) Oncology (Suppl. 1) 42:33.

Samuel, C.E. (1991) Virology 183:1.

Pellegrini, S. and C. Schindler (1993) Trends Biochem. Sci. 18:338.

Reiter, Z. (1993) J. Interferon Res. 13:247.

Boehm, U. et al. (1997) Annu. Rev. Immunol. 15:749.

Gray, P.W. et al. (1982) Nature 295:503.

Rinderknecht, E. et al. (1984) J. Biol. Chem. 259:6790.

DeGrado, W.F. et al. (1982) Nature 300:379.

Zoon, K.C. (1987) Interferon 9:1.

Ealick, S.E. et al. (1991) Science 252:698.

Lunn, C.A. et al. (1992) J. Biol. Chem. 267:17920.

Rashidbaigi, A. et al. (1986) Proc. Natl. Acad. Sci. USA 83:384.

Pfizenmaier, K. et al. (1988) J. Immunol. 141:856.

Aguet, M. et al. (1988) Cell 55:273.

Fischer, D.G. et al. (1988) J. Biol. Chem. 263:2632.

Calderon, J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4837.

Paliard, X. et al. (1988) J. Immunol. 141:849.

Christmas, S.E. (1992) Chem. Immunol. 53:32.

Locksley, R.M. and P. Scott (1991) Immunoparasitology Today A58-A61.

Billiau, A. and R. Dijkmans (1990) Biochem. Pharmacol. 40:1433.

Sen, G.C. and P. Lengyel (1992) J. Biol. Chem. 267:5017.

Gusella, G.L. et al. (1993) J. Immunol. 151:2725.15. Mirkovitch, J. et al. (1992) Mol. Cell. Biol. 12:1.

    Please let us know if you have published research using #EK0458 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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