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Human IL-23 ELISA kitEK0451

  Catalog No. Package Size Price
EK0451-1 1x96T $399.00
EK0451-2 5x96T $1799.00
EK0451-3 10x96T $3459.00

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  • Product Name Human IL-23 ELISA kit
  • Brief Description ELISA Kit
  • Applications Solid Phase Sandwich ELISA
  • Species Reactivity Human
  • Specificity Natural and recombinant Human IL-23 Ligand
  • Crossing Reactivity No significant interference observed with available related molecules.
  • Target Name Human IL-23
Application Details

Detect Range: 31.25-2000pg/ml
Sensitivity: 4pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hours
Detection method: Colorimetric

  • Human IL-23 ELISA kit - Absci

    Representative standard curve for IL-23 ELISA. IL-23 was diluted in serial two-fold steps in Sample Diluent.

Product Description
  • Aluminium pouches with a Microwell Plate coated with antibody to human IL-23 (8X12)
  • 2 vials human IL-23 Standard lyophilized, 2000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human IL-23 antibody
  • 2 vials Streptavidin-HRP solution
  • 4 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert

Interleukin 23 (IL-23) is a heterodimeric cytokine that is related to IL-12 (1-3). It is composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (3-7). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The human p19 subunit cDNA encodes a 189 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 170 aa mature protein. Human and mouse p19 subunits share 70% aa sequence identity. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor β1 subunit (IL-12 Rβ1) and the IL-23-specific receptor subunit (IL-23 R) (7). IL-23 is produced by dendritic cells and macrophages in response to pathogens including certain bacteria and viruses and/or their components (3). IL-23 and IL-12 have overlapping but distinct biological activities. The IL-23 immune pathway induces the earliest recruitment of neutrophils to the site of infection while the more classic host defense and cytotoxic response is stimulated by IL-12 (4). IL-12 drives the development of Th1 cells and induces production of IFN-γ by NK cells (3). In contrast, IL-23 has a role in the development/maintenance of a T cell subset characterized by the production of IL-17A, IL-17F, IL-6, and TNF-α (3, 4, 8). The induction of IL-17-producing T cells may involve the actions of TGF-β while their survival and expansion may be IL-23-dependent (9-11). The IL-23/IL-17 axis is an important mediator of inflammation. In mouse models, transgenic over-expression of IL-23 leads to a systemic inflammatory response (12). IL-23 effects on IL-17-producing T cells may also enhance the development of several models of autoimmune disease including experimental allergic encephalomyelitis (EAE), collagen-induced arthritis (CIA), colitis, and diabetes (5, 8, 13-17). IL-23 may also play a role in increased tumor growth associated with chronic inflammation (18). In humans, IL-23 has been found upregulated in several pathologies with dysregulated immune function including psoriasis, Crohn


Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.

Langrish, C.L. et al. (2004) Immunol. Rev. 202:96.

McKenzie, B.S. et al. (2006) Trends Immunol. 27:17.

Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Cua, D.J. et al. (2003) Nature 421:744.

Oppmann, B. et al. (2000) Immunity 13:715.

Parham, C. et al. (2002) J. Immunol. 168:5699.

Langrish, C.L. et al. (2005) J. Exp. Med. 201:233.

Veldhoen, M. et al. (2006) Immunity 24:179.

Mangan, P.R. et al. (2006) Nature 441:231.

Bettelli, E. et al. (2006) Nature 441:235.

Wiekowski, M.T. et al. (2001) J. Immunol. 166:7563.

Nakae, S. et al. (2003) J. Immunol. 171:6173.

Nakae, S. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5986.

Mensah-Brown, E.P. et al. (2006) Eur. J. Immunol. 36:216.

Yen, D. et al. (2006) J. Clin. Invest. 116:1310.

Chen, Y. et al. (2006) J. Clin. Invest. 116:1317.

Langowski, J.L. et al. (2006) Nature 442:461.

Piskin, G. et al. (2006) J. Immunol. 176:1908.

Fuss, I.J. et al. (2006) Inflamm. Bowel Dis. 12:9.

Vaknin-Dembinsky, A. et al. (2006) J. Immunol. 176:7768.

    Please let us know if you have published research using #EK0451 so that we can cite your reference.
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