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Human IL-18 ELISA kitEK0450

  Catalog No. Package Size Price
EK0450-1 1x96T $399.00
EK0450-2 5x96T $1799.00
EK0450-3 10x96T $3459.00

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  • Product Name Human IL-18 ELISA kit
  • Brief Description ELISA Kit
  • Applications Solid Phase Sandwich ELISA
  • Species Reactivity Human
  • Specificity Natural and recombinant Human IL-18 Ligand
  • Crossing Reactivity No significant interference observed with available related molecules.
  • Target Name Human IL-18
Application Details

Detect Range: 78-5000pg/ml
Sensitivity: 20pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hours
Detection method: Colorimetric

  • Human IL-18 ELISA kit - Absci

    Representative standard curve for IL-18 ELISA. IL-18 was diluted in serial two-fold steps in Sample Diluent.

Product Description
  • Aluminium pouches with a Microwell Plate coated with antibody to human IL-18 (8X12)
  • 2 vials human IL-18 Standard lyophilized, 2500pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human IL-18 antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert

Interleukin (IL) -18 is a newly discovered cytokine, structurally similar to IL-1, with profound effects on T-cell activation.

Formerly called interferon (IFN) gamma inducing factor (IGIF), IL-18 is a novel cytokine that plays an important role in the T-cell-helper type 1 (Th1) response, primarily by its ability to induce IFN gamma production in T cells and natural killer (NK) cells.

In terms of structure, IL-18 and IL-1 beta share primary amino acid sequences of the so-called "signature sequence" motif and are similarly folded as all beta pleated sheet molecules. Also similar to IL-1 beta, IL-18 is synthesized as a biologically inactive precursor molecule lacking a signal peptide which requires cleavage into an active, mature molecule by the intracellular cysteine protease called IL-1 beta-converting enzyme (ICE, caspase-1). Therefore inhibitors of ICE activity may limit the biologic activity of IL-18 and may be useful as Th1 immunosuppressive agents.

The activity of the mature IL-18 is closely related to that of IL-1.

IL-18 induces gene expression and synthesis of tumor necrosis factor (TNF), IL-1, Fas Ligand, and several chemokines.

IL-18 induces the differentiation of Th1 or Th2 cells depending upon cytokine milieu and genetic background.

The activity of IL-18 is via an IL-18 receptor (IL-18R) complex. This complex is made up of a binding chain termed IL-18R?, a member of the IL-1 receptor family previously identified as the IL-1 receptor - related protein (IL-1Rrp), and a signaling chain, also a member of the IL-1R family.

The IL-18R complex recruits the IL-1R - activating kinase (IRAK) and TNF-R - associated factor -6 (TRAF-6) with subsequent activation of NF kappa B.

Thus based on primary structure, three-dimensional structure, receptor family, signal transduction pathways and biological effects, IL-18 appears to be a new member of the IL-1 family.

Similar to IL-1, IL-18 participates in both innate and acquired immunity.

Constitutive IL-18 expression is detected from many different cells, including macrophages, keratinocytes, and osteoblasts. Human keratinocytes have been found to be the major producers of IL-18 in unprocessed form.

An important role of IL-18 has been shown in clinical implications, in the role of IL-18 modulation in tumours, infections, and autoimmune and inflammatory diseases.

Elevated serum levels of IL-18 have been shown in patients with acute graft-versus-host disease after bone marrow transplantation.

Highly elevated IL-18 bioactivity with highly elevated serum IL-18 levels are measured in hematological malignancies as well as in sepsis.

Increased concentrations of plasma IL-18 are found in patients with hepatic dysfunction after hepatectomy and in inflammatory liver diseases. Elevations of IL-18 levels accompany autoimmune disorders like systemic lupus erythematosus and rheumatoid arthritis.

Significant levels of circulating IL-18 are found in HIV-1 infection, and other infections, in inflammatory CNS diseases, in Crohn?s disease and in pregnancy during labour and complicated pregnancies.

IL-18 is regarded as a novel player in tumour immunotherapy.


Okamura H., et al. Nature 378, 88-91 (1995)

Ushio S., et al. J. Immunol. 156, 4274-4279 (1996)

Micallef M., et al. Eur. J. Immunol. 26, 1647-1651 (1996)

Tao D, et al. Cell Immunol. 173, 230-235 (1998)

M. Taniguchi, et al. J. Immunol. Methods 206, 107-113 (1997)

    Please let us know if you have published research using #EK0450 so that we can cite your reference.
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