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Human IL-5 ELISA kitEK0449

  Catalog No. Package Size Price
EK0449-1 1x96T $399.00
EK0449-2 5x96T $1799.00
EK0449-3 10x96T $3459.00

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Description
  • Product Name Human IL-5 ELISA kit
  • Brief Description ELISA Kit
  • Applications Solid Phase Sandwich ELISA
  • Species Reactivity Human
  • Specificity Natural and recombinant Human IL-5 Ligand
  • Crossing Reactivity No significant interference observed with available related molecules.
  • Target Name Human IL-5
Application Details

Detect Range: 3.9-250pg/ml
Sensitivity: 2pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hours
Detection method: Colorimetric

Images
  • Human IL-5 ELISA kit - Absci

    Representative standard curve for IL-5 ELISA. IL-5 was diluted in serial two-fold steps in Sample Diluent.

Product Description
  • Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-5 (8x12)
  • 2 vials human IL-5 Standard lyophilized, 500 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human IL-5 monoclonal antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 3 pieces Adhesive Films
  • package insert
Background

Interleukin-5(IL-5)is a secreted glycoprotein that belongs to the α-helical group of cytokines (1-3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). The cDNA for human IL-5 encodes a signal peptide and a 115 amino acid (aa) mature protein. Mature human IL-5 shares 70%, 70%, 62%, 71%, 70% and 66%, aa sequence identity with mouse, rat, canine, equine, feline and porcine IL-5, respectively and shows cross-reactivity with mouse IL-5. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin

Regerences

Rosenberg, H. F. et al. (2007) J. Allergy Clin. Immunol.119:1303.

Elsas, P.X. and M. I. G. Elsas (2007) Curr. Med. Chem.14:1925.

MartinezMoczygemba,M. and D. P. Huston (2003) J. Allergy Clin. Immunol. 112:653.

Minamitake, Y. et al. (1990) J. Biochem. 107:292.

McKenzie, A. N. et al. (1991) Mol. Immunol. 28:155.

Shakoory, B. et al. (2004) J. Interferon Cytokine Res. 24:271.

Lalani, T. et al. (1999) Ann. Allergy Asthma Immunol. 82:317.

Sakuishi, K. et al. (2007) J. Immunol. 179:3452.

Clutterbuck, E. J. et al. (1989) Blood 73:1504.

Cameron, L. et al. (2000) J. Immunol. 164:1538.

Tavernier, J. et al. (1991) Cell 66:1175.

ZaksZilberman, M. et al. (2008) J. Biol. Chem. 283:13398.

Lipscombe, R. et al. (1998) J. Leukocyte Biol. 63:342.

Tavernier, J. et al. (2000) Blood 95:1600.

Kopf, M. et al. (1996) Immunity 4:15.

Denburg, J. A. et al. (1991) Blood 77:1462.

    Please let us know if you have published research using #EK0449 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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