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Human IL-4 ELISA kitEK0448

  Catalog No. Package Size Price
EK0448-1 1x96T $399.00
EK0448-2 5x96T $1799.00
EK0448-3 10x96T $3459.00

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Description
  • Product Name Human IL-4 ELISA kit
  • Brief Description ELISA Kit
  • Applications Solid Phase Sandwich ELISA
  • Species Reactivity Human
  • Specificity Natural and recombinant Human IL-4 Ligand
  • Crossing Reactivity No significant interference observed with available related molecules.
  • Target Name Human IL-4
Application Details

Detect Range: 7.8-500pg/ml
Sensitivity: 2pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hours
Detection method: Colorimetric

Images
  • Human IL-4 ELISA kit - Absci

    Representative standard curve for IL-4 ELISA. IL-4 was diluted in serial two-fold steps in Sample Diluent.

Product Description
  • Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-4 (8x12)
  • 2 vials human IL-4 Standard lyophilized, 500 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human IL-4 polyclonal antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 3 pieces Adhesive Films
  • package insert
Background

Interleukin 4 (IL-4) is a pleiotropic cytokine produced primarily by activated T lymphocytes, mast cells and basophils (1-3). The sequence of human IL-4 cDNA predicts a 153 amino acid (aa) residue precursor protein containing a 24 aa residue signal peptide that is cleaved to form the mature protein (4). At the amino acid sequence level, mature human IL-4 is approximately 50% identical to mouse IL-4 and there is no species cross-reactivity between the two proteins (1, 2). Human IL-4 also shares approximately 30% amino acid sequence identity to human IL-13 and the two cytokines exhibit overlapping biological activities (5, 6). The gene for IL-4 has been mapped to human chromosome 5q, in close proximity to the genes for IL-3, IL-5, IL-13 and GM-CSF (1, 2).

IL-4 has multiple immune response-modulating functions on a variety of cell types. It is an important regulator of isotype switching, inducing IgE production in B lymphocytes. It is an important modulator of the differentiation of precursor T helper cells to the TH2 subset that mediates humoral immunity and modulates antibody production. In addition, IL-4 has also been shown to have anti-tumor activity both in vivo and in vitro (1-3).

The biological effects of IL-4 are mediated by specific cell surface receptor complexes. One type of functional IL-4 receptor complex consists of the IL-4-binding subunit (IL-4 R) and a second chain, designated the common c chain because it has also been identified as a component of the receptor complexes for IL-2, IL-7, IL-9 and IL-15 (7-9). A second type of functional IL-4 receptor complex, consisting of the IL-4 R and the more recently cloned IL-13 R , has also been proposed (10, 11).

Although IL-4 R does not bind IL-13 directly, it has been shown to complex with the low-affinity IL-13 R to form the functional high-affinity receptor complex for IL-13 (11, 12). In addition to the membrane-bound form of IL-4 R, a naturally occurring soluble form of IL-4 R has been identified in human and mouse biological fluids and in mouse cell culture supernates (13-15). Soluble IL-4 R has been to shown to bind IL-4 with high affinity in solution.

Regerences

Howard, M. and N. Harada (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola ed.,Oxford University Press, New York, p. 44.

Banchereau, J. and M.E. Rybak (1994) in The Cytokine Handbook, 2nd Ed., A. Thomson ed.,Academic Press, New York, p. 99.

Yokota, T. et al. (1990) in Peptide Growth Factors and Their Receptors I, Sporn, M.B. and A.B. Roberts eds., Springer-Verlag, New York, p. 577.

Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894.

McKenzie, A.N.J. and G. Zurawski (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola ed., Oxford Univ. Press, New York, p. 92.

Zurawski, G. and J.E. de Vries (1994) Immunol. Today 15:19.

Park, L.S. (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola ed., Oxford University Press, New York, p. 47.

Noguchi, M. et al. (1993) Science 262:1877.

Kondo, M. et al. (1993) Science 262:1874.

Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:497.

Lin, J-X. et al. (1995) Immunity 2:331.

Zurawski, S.M. et al. (1995) J. Biol. Chem. 270:13869.

Fanslow, W.C. et al. (1990) Cytokine 2:398.

Fernandez-Botran, R. et al. (1990) Proc. Natl. Acad. Sci. USA 87:4202.

15. Ruhl, S. et al. (1993) Cytokine 5:144.

    Please let us know if you have published research using #EK0448 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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