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Human IL-13 ELISA kitEK0447

  Catalog No. Package Size Price
EK0447-1 1x96T $399.00
EK0447-2 5x96T $1799.00
EK0447-3 10x96T $3459.00

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  • Product Name Human IL-13 ELISA kit
  • Brief Description ELISA Kit
  • Applications Solid Phase Sandwich ELISA
  • Species Reactivity Human
  • Specificity Natural and recombinant Human IL-13 Ligand
  • Crossing Reactivity No significant interference observed with available related molecules.
  • Target Name Human IL-13
Application Details

Detect Range: 3.125-200pg/ml
Sensitivity: 1pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hours
Detection method: Colorimetric

  • Human IL-13 ELISA kit - Absci

    Representative standard curve for IL-13 ELISA. IL-13 was diluted in serial two-fold steps in Sample Diluent.

Product Description
  • Aluminium pouches with a Microwell Plate coated with antibody to human IL-13 (8X12)
  • 2 vials human IL-13 Standard lyophilized, 200 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human IL-13 antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert

Interleukin-13 (IL-13) is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis (1-3). It is secreted by Th1, Th2, Th17, NK, and mast cells, visceral smooth muscle cells, eosinophils, and basophils (2, 4). This pattern is similar to the expression of Interleukin-4 (IL-4) but also includes subsets of Th1 and Th17 cells that do not secrete IL-4 (5). IL-13 circulates as a monomer and has two internal disulfide bonds that contribute to its bundled four α-helix configuration (6, 7). Mature human IL-13 shares approximately 58% amino acid sequence identity with mouse and rat IL-13. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (8, 9). IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. On B cells, it promotes cellular activation, immunoglobulin class switching to IgE, and the upregulation of CD23/Fcε RII. Polymorphisms and upregulation of IL-13 are associated with atopy, asthma, airway hyperresponsiveness, and tissue fibrosis (1, 10).

The biological effects of IL-13 and IL-4 are closely related due in part to a shared receptor system. IL-13 binds with low affinity to the transmembrane IL-13 Rα1 which then forms a signaling complex with the transmembrane IL-4 Rα (11-13). This high affinity receptor complex also functions as the type 2 IL-4 receptor (11, 12). Soluble forms of IL-4 Rα are expressed which retain ligand binding properties and inhibit IL-4 bioactivity (14, 15). IL-4 Rα also associates with the common gamma chain (γc) to form the type 1 IL-4 receptor complex (16, 17).

Additionally, IL-13 binds with high affinity to IL-13 Rα2 which is expressed as cell surface and soluble forms (18-20). IL-13 Rα2 functions as a decoy receptor by preventing IL-13 from signaling through the IL-13 Rα1/IL-4 Rα complex (21, 22). It also inhibits responsiveness to IL-4 by blocking signaling through IL-4-occupied IL-13 Rα1/IL-4 Rα receptor complexes (22, 23). IL-13 Rα2 is upregulated during Th2-biased immune responses and limits inflammatory tissue damage (20, 24, 25). Aside from its decoy function, IL-13 Rα2 can signal in response to IL-13 to directly promote tumor cell invasiveness and the development of tissue fibrosis (26-28).


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