Welcome     Login   Register   Message Board   
Your Position: Home > ELISA Kits > Human IL-1β ELISA kit

Human IL-1β ELISA kitEK0445

  Catalog No. Package Size Price
EK0445-1 1x96T $399.00
EK0445-2 5x96T $1799.00
EK0445-3 10x96T $3459.00

Quantity:  Total:   

Review:comment rank 5 pdf

Availability: promotionThe lead time is currently 1 week.

add promotion
  • Product Name Human IL-1β ELISA kit
  • Brief Description ELISA Kit
  • Applications Solid Phase Sandwich ELISA
  • Species Reactivity Human
  • Specificity Natural and recombinant Human IL-1β Ligand
  • Crossing Reactivity No significant interference observed with available related molecules.
  • Target Name Human IL-1β
Application Details

Detect Range: 7.8-500pg/ml
Sensitivity: 2pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hours
Detection method: Colorimetric

  • Human IL-1β ELISA kit - Absci

    Representative standard curve for IL-1 ELISA. IL-1 was diluted in serial two-fold steps in Sample Diluent.

Product Description
  • Aluminium pouches with a Microwell Plate coated with antibody to human IL-1β (8x12)
  • 2 vials human IL-1β Standard lyophilized, 500 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human IL-1β antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert

Interleukin-1 (IL-1), originally described as lymphocyte activating factor (LAF) for its effects on thymocytes(1), is a polypeptide cytokine with two molecular forms. The two distinct molecular forms of IL-1 are thought to be derived from two genes(2,3). After transcription, as 31kD precursor polypeptide is cleaved to give rise to mostly cell membrane associated IL-1? and secreted IL-1?. Both have the same molecular weight of 15kD but have different isoelectric points of 5 and 7, respectively.

Despite sequence homology of only 20%, both forms are thought to bind to the same receptor(4). IL-1 inhibitors that vary only in their degree of glycosylation have been described to bind to the IL-1 receptor(5). These inhibitors are structurally related to IL-1β and may be important in regulation of IL-1? action(6).

IL-1? is produced primarily by monocytes and macrophages (7) but also by astrocytes, oligodendroglia, adrenal cortical cells, NK cells, endothelial cells, keratinocytes, megakaryocytes, platelets, neurons, neutrophils, osteoblasts, Schwann cells, trophoblasts, T cells, and fibroblasts. IL-1 has multiple immunological functions including enhancement of IL-2 production by T cells and activation of B-cells (BAF) and thymocytes(8-10). A true pleiotrope, IL-1 may have tumoricidal activity via its release of IL-2 and Interferon gamma and indirectly antiviral by stimulating to release interferon beta(11).

Low levels of IL-1? have been reported in normal serum(12). It is thought that IL-1 genes are induced to respond to tissue damage or in infection. Elevated levels have been reported in a number of infectious disease conditions and in noninfectious inflammatory conditions such as Crohn


Gery L.,et al.(1972). J Exp Med 136, 128.

March CJ., et al.(1985). Nature 315,641.

Gray PW., et al.(1986). J Immunol 137, 3644.

Dinarello CA.(1989). Adv Immunol 44,153.

Hannum CH., et al.(1990). Nature 343,336.

Eisenberg SP., et al.(1990). Nature 343,341.

Wewers, M.D. et al. (1997) J. Immunol. 159:5964.

Oppenheim JJ., et al.(1986).Immunol Today7,45.

Dinarello CA., et al.(1986). J Clin Invest77,1734.

FalkoffRJ., et al.(1984). J Immunol 133,692.

Ben-Aribia MH., et al.(1987). J Immunol139,443.

Gahring LC.(1984). Fed Proc 43,462.

Eastgate JA., et al.(1988).Lancet2,706.

Bodel PT.,et al.(1986).Br J Exp Pathol 49,11.

    Please let us know if you have published research using #EK0445 so that we can cite your reference.
Customer Reviews and Rankings
  • No comment
  • Total 0 results, divided into1 pages. First Prev Next Last

    My Review

    Username: Anonymous user
    Verification code: captcha
    Browsing historyclear
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  

    © 2017, AbSci All Rights Reserved. E-mail: info@abscitech.com
    苏B1-20150380 苏ICP备15009006号-1