PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of Zap-70 only when phosphorylated at tyrosine 493.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 493 (S-Y-Y(p)-T-A) derived from Human Zap-70.
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 70kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Images
Western blot analysis of extracts from JK cells untreated or treated with anisomycin using Zap-70(Phospho-Tyr493) Antibody #AB11160.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Zap-70(Phospho-Tyr493) Antibody #AB11160(left) or the same antibody preincubated with blocking peptide(right).
Background
Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Chan AC, et al. (1995) EMBO J; 14(11): 2499-2508
Kong G, et al. (1996) Mol Cell Biol; 16(9): 5026-5035
Salomon AR, et al. (2003)Proc Natl Acad Sci U S A; 100(2): 443-448