PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of JAK1 only when phosphorylated at tyrosine 1022.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 1022 (K-E-Y(p)-Y-T) derived from Human JAK1.
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 130kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Images
Western blot analysis of extracts from HT29 cells untreated or treated with UV using JAK1(Phospho-Tyr1022) Antibody #AB11149.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JAK1(Phospho-Tyr1022) Antibody #AB11149(left) or the same antibody preincubated with blocking peptide(right).
Background
Tyrosine kinase of the non-receptor type, involved in the IFN-a/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
Zheng H, et al.(2005)Mol Cell Proteomics. 4(6):721-730.
Wang R, et al.(2003) Arch Biochem Biophys. 410(1): 7-15.