PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of FAK only when phosphorylated at tyrosine 925.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 925 (K-V-Y(p)-E-N) derived from Human FAK.
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 125kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Images
Western blot analysis of extracts from 293 cells using FAK(Phospho-Tyr925) Antibody #AB11123(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Immunofluorescence staining of methanol-fixed Hela cells using FAK(Phospho-Tyr925) Antibody #AB11123.
Background
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Sanders MA, et al. (2005) J Biol Chem; 280(25): 23516-22.
Cherubini A, et al. (2005) Mol Biol Cell; 16(6): 2972-83.
Toriumi Y, et al. (2003) FEBS Lett; 553(3): 419-22.