ATM(Phospho-Ser1981) Antibody

Description

Product Name ATM(Phospho-Ser1981) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu
Specificity The antibody detects endogenous level of ATM only when phosphorylated at serine 1981.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 1981 (E-G-S(p)-Q-S) derived from Human ATM.
Target Name ATM
Modification Phospho-Ser1981
Alternative Names Ataxia telangiectasia mutated homolog; Ataxia telangiectasia mutated; kinase ATM
Accession No. Swiss-Prot: Q13315
NCBI Protein: NP_000042.3
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 350kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from Hela cells untreated or treated with UV using ATM(Phospho-Ser1981) Antibody #AB11122
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATM(Phospho-Ser1981) Antibody #AB11122(left) or the same antibody preincubated with blocking peptide(right).

Background

ATM encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene.

Gupta A. et al. (2005) Mol Cell Biol. 25(12): 5292-5305.
Bernstein JL. et al. (2002) Breast Cancer Res. 4(6): 249-252.
Silverman J. et al. (2004) Genes Dev. 18(17): 2108-2119.
Nakada D. et al. (2003) Nucleic Acids Res. 31(6): 1715-1724.

Published Papers

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