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BRCA1(Phospho-Ser1524) AntibodyAB11117

  Catalog No. Package Size Price
AB11117-1 50ul $189.00
AB11117-2 100ul $279.00
AB11117-4 25ul $119.00

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Description
  • Product Name BRCA1(Phospho-Ser1524) Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
  • Applications IHC
  • Species Reactivity Hu
  • Specificity The antibody detects endogenous level of BRCA1 only when phosphorylated at serine 1524.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around phosphorylation site of serine 1524 (Y-P-S(p)-Q-E) derived from Human BRCA1.
  • Target Name BRCA1
  • Modification Phospho-Ser1524
  • Alternative Names RNF53
  • Accession No. Swiss-Prot: P38398
    NCBI Protein: NP_009225.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 220kd
Immunohistochemistry: 1:50~1:100

Images
  • BRCA1(Phospho-Ser1524) Antibody - Absci

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BRCA1(Phospho-Ser1524) Antibody #AB11117(left) or the same antibody preincubated with blocking peptide(right).

Background
The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation

Lin SY, et al. (2004) Proc Natl Acad Sci U S A, 101(17): 6484-6489
Zhang J, et al. (2004) Mol Cell Biol; 24(2): 708-718
Beger C, et al. (2001) Proc Natl Acad Sci U S A; 98(1): 130-135
Gardner K, et al. (2001) Breast Cancer Res; 3(1): 11-13
Zheng L, et al. (2001) Proc Natl Acad Sci U S A; 98(17): 9587-9592
    Please let us know if you have published research using #AB11117 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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