PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of Tau only when phosphorylated at serine 235.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around phosphorylation site of serine 235 (P-K-S(p)-P-S) derived from Human Tau.
Target NameTau
ModificationPhospho-Ser235
Alternative NamesMAPT; MTAPT; MTBT1; Neurofibrillary tangle protein; PHF-tau
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 48 62 78 kd
Western blotting: 1:500~1:1000
Images
Western blot analysis of extracts from mouse brain tissue using Tau(Phospho-Ser235) Antibody #AB11106 and the same antibody preincubated with blocking peptide.
Background
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Alonso Adel C, et al. (2004) J Biol Chem. 279(33): 34873-34881.
Liu F, et al. (2002) FEBS Lett. 530(1-3): 209-214.
Sengupta A, et al. (1998) Arch Biochem Biophys. 357(2): 299-309.