PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of Caveolin-1 only when phosphorylated at tyrosine 14.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 14 (H-L-Y(p)-T-V) derived from Human CAVEOLIN-1.
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 24kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Images
Western blot analysis of extracts from 3T3 cells untreated(lane 1) or treated with H2O2(lane 2) using Caveolin-1(Phospho-Tyr14) Antibody #AB11090.
Immunofluorescence staining of methanol-fixed Hela cells using Caveolin-1(Phospho-Tyr14) Antibody #AB11090.
Background
The scaffolding protein encoded by Caveolin-1 is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (a and beta) are encoded by a single transcript from this gene.
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Maggi D, et al. (2002) Biochem Biophys Res Commun. 295(5): 1085-1089.