PurificationAffinity purification using immunogen.
ApplicationsWB
Species ReactivityVarious Plants
SpecificityAntibody detects endogenous various plants actin protein.
Target NamePlant actin
SDS-PAGE MW45kd
Concentration1.0mg/ml
FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:3000~1:5000
Images
Western blot analysis of Arabidopsis, using #AB38088 diluted at 1) 1:5,000 2) 1:10,000.
Background
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence. Antibodies against plant Actin are useful as loading controls for Western Blotting.