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β-actin Mouse Monoclonal AntibodyAB38069

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Description

Product Name β-actin Mouse Monoclonal Antibody
Host Species Mouse
Clonality Monoclonal
Purification Affinity purification using immunogen.
Applications WB
Species Reactivity Hu Rt Ms Mk Dg
Specificity Antibody detects endogenous β-actin protein.
Target Name β-actin
Alternative Names ACTB; BRWS1; PS1TP5BP1
Accession No. Swiss-Prot#:P60709
SDS-PAGE MW 45kd
Concentration 1.0mg/ml
Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C

Application Details

Western blotting: 1:1000~1:3000

Images

Western blot analysis of 1) Hela, 2) Mouse Brain tissue, 3) Rat Brain tissue, using #AB38069 diluted at 1:5,000.

Background

β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.

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