PurificationAffinity purification using immunogen.
ApplicationsWB
Species ReactivityZebrafish
SpecificityThe α-tubulin antibody can detects Zebrafish endogenousα-tubulin protein..
Target Nameα tubulin
Alternative Namesalpha-tubulin N-acetyltransferase; ATAT1; C6orf134; CF134; chromosome 6 open reading frame 134; DKFZp547J097; FLJ13158; LOC79969; MEC-17; MEC17; Nbla00487
Accession No.Swiss-Prot#:Q5SQI0
SDS-PAGE MW52kd
Concentration1.0mg/ml
FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:5000~1:10000
Images
Western blot analysis of Zebrafish skeletal muscle, using #AB38059 diluted at 1:5,000.
Background
Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 KD. Microtubules are assembled from dimers of α- and β-tubulin.