PurificationAffinity purification using immunogen.
ApplicationsWB IF IP
Species ReactivityHu Rt Ms
SpecificityThe Histone H3 antibody can detects endogenous Histone H3 protein.
Target NameHistone H3
Alternative NamesH3/A; H3FA;H3FB; H3FC
Accession No.Swiss-Prot#:P68431
SDS-PAGE MW17kd
Concentration1.0mg/ml
FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:2000~1:5000 Immunofluorescence: 1:100-500 Immunoprecipitation: 1:200
Images
Western blot analysis of 1) Hela, 2) Raw, 3) Mouse Brain Tissue, 4) Rat Brain Tissue, using #AB37988 diluted at 1:5,000.
IF analysis of Hela with DAPI (Left) and #AB37988(Right) diluted at 1:200.
1¡¢Input: Hela Cell Lysate 2¡¢IP product: IP dilute 1:200 Western blot analysis: primary antibody : #AB37988 1:2,000 Secondary antibody: Goat anti-Mouse IgG(H+L) 1:10,000.
Background
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.