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PCNA Mouse Monoclonal AntibodyAB37978

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Description

Product Name PCNA Mouse Monoclonal Antibody
Host Species Mouse
Clonality Monoclonal
Purification Affinity purification using immunogen.
Applications WB IHC
Species Reactivity Hu Ms
Specificity Antibody can detect endogenous?PCNA protein.
Target Name PCNA
SDS-PAGE MW 36kd
Concentration 1.0mg/ml
Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C

Application Details

Western blotting: 1:5000
Immunohistochemistry: 1:200

Images

Western blot analysis of Hela (lane 1), Rat brain (lane 2), NIH 3T3 (lane 3), 293T (lane 4), using #AB37978 diluted at 1:5000.
IHC staining of paraffin- embedded Human breast cancer tissue with PCNA mouse mAb (12D10) diluted at 1:200.

Background

PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

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