HER2(Phospho-Tyr1248) Antibody

Description

Product Name HER2(Phospho-Tyr1248) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC IF
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of HER2 only when phosphorylated at tyrosine1248.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine1248 (P-E-Y(p)-L-G) derived from Human HER2.
Target Name HER2
Modification Phospho-Tyr1248
Alternative Names C-erbB-2; ErbB2;
Accession No. Swiss-Prot: P04626
NCBI Protein: NP_001005862.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 185kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2 (Phospho-Tyr1248) Antibody #AB11079 (left) or the same antibody preincubated with blocking peptide #BP51079 (right).
Immunofluorescence staining of methanol-fixed MCF7 cells using HER2(Phospho-Tyr1248) Antibody #AB11079.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using HER2 (Phospho-Tyr1248) Antibody #AB11079.
Western blot analysis of extracts from HepG2 cells treated with PMA and MCF cells treated with EGF, using HER2 (Phospho-Tyr1248) Antibody #AB11079.

Background

Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin.

Yuan,Lu Qian ,Ming Shi, et al. (2008) HER2-dependent MMP-7 expression is mediated by activated STAT3 Guogang Cellular Signalling, 20:1284

Published Papers

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