HER2(Phospho-Tyr1221/Tyr1222) Antibody

Description

Product Name HER2(Phospho-Tyr1221/Tyr1222) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IF
Species Reactivity Human
Specificity The antibody detects endogenous level of HER2 only when phosphorylated at tyrosine1221/tyrosine1222.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of tyrosine1221/1222 (N-L-Y(p)-Y(p)-W) derived from Human HER2.
Target Name HER2
Modification Phospho-Tyr1221/Tyr1222
Alternative Names C-erbB-2; ErbB2;
Accession No. Swiss-Prot: P04626
NCBI Protein: NP_001005862.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 185kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from MDA cells untreated or treated with EGF using HER2(Phospho-Tyr1221/Tyr1222) Antibody #AB11076.
Immunofluorescence staining of methanol-fixed MCF cells using HER2(Phospho-Tyr1221/Tyr1222) Antibody #AB11076.
Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using HER2 (Phospho-Tyr1221/Tyr1222) Antibody #AB11076.

Background

Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin.

Marone R, et al. (2004) Nat Cell Biol; 6(6): 515-22.
Ren Z, et al. (2002) J Biol Chem; 277(41): 38486-93.

Published Papers

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