Estrogen Receptor-a(Phospho-Ser167) Antibody

Description

Product Name Estrogen Receptor-a(Phospho-Ser167) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC IF
Species Reactivity Hu
Specificity The antibody detects endogenous level of Estrogen Receptor-a only when phosphorylated at serine16.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 167 (L-A-S(p)-T-N) derived from Human Estrogen Receptor-a.
Target Name Estrogen Receptor-a
Modification Phospho-Ser167
Alternative Names ER; ESR; ESR1; ESTR; ESTRA
Accession No. Swiss-Prot: P03372
NCBI Protein: NP_000116.2
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 66kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from MCF7 cells using Estrogen Receptor-a(Phospho-Ser167) Antibody #AB11073(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-a(Phospho-Ser167) Antibody #AB11073(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF cells using Estrogen Receptor-a(Phospho-Ser167) Antibody #AB11073.

Background

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.

Feinmesser RL,et al. (1999) J Biol Chem; 274(23): 16168-73.
Gamou S,et al. (1995)FEBS Lett; 357(2): 161-4.
Gamou S, et al. (1994)J Cell Physiol; 158(1): 151-9.
Heisermann GJ, et al. (1988) J Biol Chem; 263(26): 13152-8.

Published Papers

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