BAD(Phospho-Ser112) Antibody

Description

Product Name BAD(Phospho-Ser112) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu Ms
Specificity The antibody detects endogenous level of BAD only when phosphorylated at serine 112.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 112 (H-S-S(p)-Y-P) derived from Mouse BAD.
Target Name BAD
Modification Phospho-Ser112
Alternative Names Bbc2
Accession No. Swiss-Prot: Q61337
NCBI Protein: NP_031548.1
SDS-PAGE MW 23kd
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 23kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from cos7 cells untreated or treated with EGF using BAD(Phospho-Ser112) Antibody #AB11067
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD(Phospho-Ser112) Antibody #AB11067(left) or the same antibody preincubated with blocking peptide(right).

Background

The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Zhang B, et al. (2004). Mol Cell Biol.24 (14): 6205-6214.
Rice PL, et al. (2003). Cancer Res.63 (3): 616-620.
Wang XQ, et al. (2001). J Biol Chem.276 (48): 44504-44511.

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