Immunofluorescence (IF)

Immunofluorescence (IF) is used to analyze the sub-cellular localization of a protein within a cell. This technique usually uses a labeled antibody to target the fluorescent dye to its antigen. For the emitted light by the fluorescent dye can be detected in fluorescence microscope, this allows visualization of the distribution of a target protein in the sample. AbSci follows stringent validation protocol to ensure IF application.

Immunofluorescence (IF) validation methods:

Using appropriate cell lines for analysis

Stimulus treatment

Inhibitor treatment

Using appropriate cell lines for analysis

Immunofluorescence analysis of methanol-fixed MCF7 cells using HER2 (Phospho-Tyr1248) Antibody #AB11079.

Immunofluorescence analysis of methanol-fixed Hela cells using GSK3β (Phospho-Ser9) Antibody #AB11002.

Immunofluorescence analysis of methanol-fixed MEF cells using Histone H3 (Tri-Methyl-Lys27) Antibody #AB11582.

Stimulus treatment

Immunofluorescence analysis of methanol-fixed MEF cells untreated or treated with IFN using STAT3 (Phospho-Tyr705) Antibody #AB11045.

Inhibitor treatment

Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with LY2904 using FKHR (Phospho-Ser256) Antibody #AB11115.

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