New Phospho-specificity Verification with Phosphatase

In order to further verify phospho-specificity and ensure quality, AbSci has increased phosphatase treatment method in validation of phospho-specific antibodies. Up to the present AbSci has used numerous analytical methods in antibody characterization.
         Western blotting(WB)
                  Phosphatase treatment
                  Stimulus treatment
                  Blocking peptide control
                  Loading control
                  Validation by various cell lines
         Immunohistochemistry(IHC)
                  Blocking peptide control
                  Validation with different tissues
         Immunofluorescence / Immunocytochemistry(IF/ICC)
                 Validation by various cell lines
                 Subcellular localization and co-localization analysis
         Flow cytometry(FCM)   
         ELISA

 

	Akt(Phospho-Ser473) Antibody AB11054
	Western blot analysis of extracts from 293 cells, treatedwith EGF or calf intestinal phosphatase (CIP), using Akt(Phospho-Ser473) Antibody #AB11054.
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	PTEN(Phospho-Ser380/Thr382/Thr383) Antibody AB11056
	Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody #AB11056.
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	Tau(Phospho-Ser404) Antibody AB11112
	Western blot analysis of extracts from C6 cells, treated with calf intestinal phosphatase (CIP), using Tau (Phospho-Ser404) Antibody #AB11112.
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	NFkB-p65(Phospho-Ser529) Antibody AB11217
	Western blot analysis of extracts from MCF cells, treated with TNFa+CA or calf intestinal phosphatase (CIP), using NFκB-p65 (Phospho-Ser529) Antibody #AB11217.
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	mTOR(Phospho-Ser2448) Antibody AB11221
	Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using mTOR (Phospho-Ser2448) Antibody #AB11221.
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	GSK3α (Phospho-Ser21) Antibody AB11007
	Western blot analysis of extracts from 293 cells, treated with insulin or calf intestinal phosphatase (CIP), using GSK3α (Phospho-Ser21) Antibody #AB11007.
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	ATF2 (Phospho-Thr73 or 55) Antibody AB11032
	Western blot analysis of extracts from COS7 cells, treated with UV or calf intestinal phosphatase (CIP), using ATF2 (Phospho-Thr73 or 55) Antibody #AB11032.
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	MEF2A (Phospho-Thr312) Antibody AB11039
	Western blot analysis of extracts from C2C12 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using MEF2A (Phospho-Thr312) Antibody #AB11039.
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	STAT1 (Phospho-Tyr701) Antibody AB11044
	Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using STAT1 (Phospho-Tyr701) Antibody #AB11044.
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	STAT3 (Phospho-Ser727) Antibody AB11046
	Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using STAT3 (Phospho-Ser727) Antibody #AB11046.
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	STAT6 (Phospho-Thr645) Antibody AB11051
	Western blot analysis of extracts from Hela cells, treated with IL-4 or calf intestinal phosphatase (CIP), using STAT6 (Phospho-Thr645) Antibody #AB11051.
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	Estrogen Receptor-α (Phospho-Ser118) Antibody AB11072
	Western blot analysis of extracts from COS7 cells, treated with starve or calf intestinal phosphatase (CIP), using Estrogen Receptor-α (Phospho-Ser118) Antibody #AB11072.
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	HER2 (Phospho-Tyr1221/Tyr1222) Antibody AB11076
	Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using HER2 (Phospho-Tyr1221/Tyr1222) Antibody #AB11076.
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	ICAM-1 (Phospho-Tyr512) Antibody AB11083
	Western blot analysis of extracts from C2C12 cells, treated with starve or calf intestinal phosphatase (CIP), using ICAM-1 (Phospho-Tyr512) Antibody #AB11083.
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	p53 (Phospho-Ser33) Antibody AB11097
	Western blot analysis of extracts from HT29 cells, treated with serum or calf intestinal phosphatase (CIP), using p53 (Phospho-Ser33) Antibody #AB11097.
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	p53 (Phospho-Ser46) Antibody AB11099
	Western blot analysis of extracts from 293 cells, treated with UV+serum or calf intestinal phosphatase (CIP), using p53 (Phospho-Ser46) Antibody #AB11099.
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	p90RSK (Phospho-Ser352) Antibody AB11113
	Western blot analysis of extracts from Hela cells, treated with PMA or calf intestinal phosphatase (CIP), using p90RSK (Phospho-Ser352) Antibody #AB11113.
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	Androgen Receptor (Phospho-Ser650) Antibody AB11120
	Western blot analysis of extracts from 293 cells, treated with PMA or calf intestinal phosphatase (CIP), using Androgen Receptor (Phospho-Ser650) Antibody #AB11120.
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	IKK α (Phospho-Thr23) Antibody AB11129
	Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using IKK α (Phospho-Thr23) Antibody #AB11129.
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	Chk1 (Phospho-Ser280) Antibody AB11140
	Western blot analysis of extracts from A2780 cells, treated with calf intestinal phosphatase (CIP), using Chk1 (Phospho-Ser280) Antibody #AB11140.
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	Src(Phospho-Tyr529) Antibody AB11153
	Western blot analysis of extracts from HepG2 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using Src (Phospho-Tyr529) Antibody #AB11153.
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	Zap-70 (Phospho-Tyr319) Antibody AB11159
	Western blot analysis of extracts from HL60 cells, treated with calf intestinal phosphatase (CIP), using Zap-70 (Phospho-Tyr319) Antibody #AB11159.
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	MEK1 (Phospho-Ser221) Antibody AB11161
	Western blot analysis of extracts from Hela cells, treated with calf intestinal phosphatase (CIP), using MEK1 (Phospho-Ser221) Antibody #AB11161.
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	HSP27 (Phospho-Ser15) Antibody AB11164
	Western blot analysis of extracts from Hela cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using HSP27 (Phospho-Ser15) Antibody #AB11164.
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	AMPKα1 (Phospho-Ser487)Antibody AB11174
	Western blot analysis of extracts from C6 cells, treated with EGF or calf intestinal phosphatase (CIP), using AMPKα1 (Phospho-Ser487) Antibody #AB11174.
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	EGFR (Phospho-Thr693) Antibody AB11187
	Western blot analysis of extracts from HUVEC cells, treated with starve or calf intestinal phosphatase (CIP), using EGFR (Phospho-Thr693) Antibody #AB11187.
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	APP (Phospho-Thr668) Antibody AB11190
	Western blot analysis of extracts from Hela cells, treated with Noc or calf intestinal phosphatase (CIP), using APP (Phospho-Thr668) Antibody #AB11190.
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	HDAC2 (Phospho-Ser394) Antibody AB11191
	Western blot analysis of extracts from HepG2 cells, treated with calf intestinal phosphatase (CIP), using HDAC2 (Phospho-Ser394) Antibody #AB11191.
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	HSF1 (Phospho-Ser307) Antibody AB11195
	Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using HSF1 (Phospho-Ser307) Antibody #AB11195.
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	Stathmin 1 (Phospho-Ser16) Antibody AB11234
	Western blot analysis of extracts from Hela cells, treated with Noc or calf intestinal phosphatase (CIP), using Stathmin 1 (Phospho-Ser16) Antibody #AB11234.
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	CDC2 (Phospho-Tyr15) Antibody AB11244
	Western blot analysis of extracts from Hela cells, treated with Noc or calf intestinal phosphatase (CIP), using Stathmin 1 (Phospho-Ser16) Antibody #AB11244.
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	HSP27 (Phospho-Ser82) Antibody AB11248
	Western blot analysis of extracts from Hela cells, treated with UV or calf intestinal phosphatase (CIP), using HSP27 (Phospho-Ser82) Antibody #AB11248.
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	P38 MAPK (Phospho-Thr180) Antibody AB11252
	Western blot analysis of extracts from HL60 cells, treated with TNFa or calf intestinal phosphatase (CIP), using P38 MAPK (Phospho-Thr180) Antibody #AB11252.
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	HSF1 (phospho-Ser303) Antibody AB11263
	Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using HSF1 (phospho-Ser303) Antibody #AB11263.
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	Merlin (Phospho-Ser518) Antibody AB11266
	Western blot analysis of extracts from C6 cells, treated with calf intestinal phosphatase (CIP), using Merlin (Phospho-Ser518) Antibody #AB11266.
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	Cortactin (Phospho-Tyr466) Antibody AB11272
	Western blot analysis of extracts from JK cells, treated with H2O2 or calf intestinal phosphatase (CIP), using Cortactin (Phospho-Tyr466) Antibody #AB11272.
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	p62Dok (phospho-Tyr362) Antibody AB11276
	Western blot analysis of extracts from JK cells, treated with G-CSF or calf intestinal phosphatase (CIP), using p62Dok (phospho-Tyr362) Antibody #AB11276.
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	p56Dok-2 (Phospho-Tyr299) Antibody AB11278
	Western blot analysis of extracts from JK cells, treated with G-CSF or calf intestinal phosphatase (CIP), using p56Dok-2 (Phospho-Tyr299) Antibody #AB11278.
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	PKCθ (Phospho-Ser676) Antibody AB11297
	Western blot analysis of extracts from PC12 cells, treated with PMA or calf intestinal phosphatase (CIP), using PKCθ (Phospho-Ser676) Antibody #AB11297.
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	IκB-β (Phospho-Ser23) Antibody AB11304
	Western blot analysis of extracts from HT29 cells, treated with CA or calf intestinal phosphatase (CIP), using IκB-β (Phospho-Ser23) Antibody #AB11304.
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	Keratin 8 (Phospho-Ser74) Antibody AB11307
	Western blot analysis of extracts from 293 cells, treated with calf intestinal phosphatase (CIP), using Keratin 8 (Phospho-Ser74) Antibody #AB11307.
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	Myc (Phospho-Ser62) Antibody AB11311
	Western blot analysis of extracts from K562 cells, treated with calf intestinal phosphatase (CIP), using Myc (Phospho-Ser62) Antibody #AB11311.
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	SHP-2 (Phospho-Tyr580) Antibody AB11320
	Western blot analysis of extracts from 293 cells, treated with starve or calf intestinal phosphatase (CIP), using SHP-2 (Phospho-Tyr580) Antibody #AB11320.
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	α-catenin (Phospho-Ser641) Antibody AB11330
	Western blot analysis of extracts from HT29 cells, treated with serum or calf intestinal phosphatase (CIP), using α-catenin (Phospho-Ser641) Antibody #AB11330.
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	JNK1/JNK2/JNK3 (phospho-Thr183/Tyr185) Antibody AB11504
	Western blot analysis of extracts from 293 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using JNK1/JNK2/JNK3 (phospho-Thr183/Tyr185) Antibody #AB11504.
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	MARCKS (phospho-Ser170) Antibody AB11535
	Western blot analysis of extracts from 3T3 cells, treated with serum or calf intestinal phosphatase (CIP), using MARCKS (phospho-Ser170) Antibody #AB11535.
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	Lck (phospho-Tyr505) Antibody AB11537
	Western blot analysis of extracts from 293 cells, treated with calf intestinal phosphatase (CIP), using Lck (phospho-Tyr505) Antibody #AB11537.
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	NFκB-p105/p50(Phospho-Ser337) Antibody AB11017
	Western blot analysis of extracts from Hela cells, treated with Noc or calf intestinal phosphatase (CIP), using NFκB-p105/p50(Phospho-Ser337) Antibody #AB11017.
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