GFP Antibody

Description

Product Name GFP Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antigen affinity purification.
Applications WB
Specificity The antibody detects transfected proteins contanining GFP tag.
Immunogen Type Recombinant Protein
Immunogen Description Full length fusion protein
Target Name GFP
Alternative Names GFP-like chromoprotein
Accession No. Gene Accssion: P42212
SDS-PAGE MW 27kd
Concentration 3.2mg/ml
Formulation Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Storage Store at -20˚C

Application Details

Western blotting: 1:1000-1:10000

Images

Gel: 8%SDS-PAGE
Lysate: 20ug GFP transfected 293T cell
Primary antibody: 1/800 dilution
Secondary antibody dilution: 1/8000
Exposure time: 30 seconds
Gel: 8%SDS-PAGE
Lysates: 20ug GFP transfected 293T cell per lane
Primary antibody: at various dilution
Secondary antibody dilution: 1/8000
Exposure time: 30 seconds

Background

Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.

Published Papers

let us know

Customer Reviews and Rankings

No comment