ACTIN Antibody

Description

Product Name ACTIN Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antigen affinity purification.
Applications WB
Species Reactivity Plants
Specificity The antibody detects endogenous levels of total ACTIN protein.
Immunogen Type Peptide
Immunogen Description Synthetic peptide corresponding to residues near the N terminal of Arabidopsis thaliana actin
Target Name ACTIN
Alternative Names ACTB
Accession No. Swiss-Prot#: P53497
NCBI Gene ID: 823805
Gene Accssion: NP_190236.1
SDS-PAGE MW 42kd
Concentration 1mg/ml
Formulation Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Storage Store at -20˚C

Application Details

Western blotting: 1:500-1:2000

Images

Gel: 10%SDS-PAGE
Lysates (from left to right): Cinnamomum camphora leaves, Nicotiana tabacum leaves, Triticum aestivuml leaves, Arabidopsis thaliana leaves, Brassica campestris flowers, Brassica campestris leaves, Vicit Faba leaves
Amount of lysate: 50ug per lane
Primary antibody: 1/500 dilution
Secondary antibody dilution: 1/8000
Exposure time: 20 seconds

Background

Member of actin subclass composed of ACT12 and ACT4. RNA is expressed at very low levels in vegetative organs, low levels in flowers and very high levels in pollen. Expression of an ACT12/GUS fusion was found in vascular tissues, tapetum, developing and mature pollen, the root cap and in a ring of pericycle tissues during lateral root initiation and early development.

Published Papers

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