PKM2 Antibody

Description

Product Name PKM2 Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IF
Species Reactivity Hu
Specificity The antibody detects endogenous levels of total PKM2 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.405~409(T-S-D-P-T) derived from Human PKM2.
Target Name PKM2
Alternative Names PKM; PK3; OIP3; PK2;
Accession No. Swiss-Prot: P14618-1
NCBI Protein: NP_872270.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 60kd
Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from HepG2 and 293 cells using PKM2 Antibody #AB21578.
Immunofluorescence staining of methanol-fixed MEF cells using PKM2 Antibody #AB21578.

Background

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Christofk, H.R. et al. (2008) Nature 452, 230-3.
Mazurek, S. et al. (2005) Semin Cancer Biol 15, 300-8.
Dombrauckas, J.D. et al. (2005) Biochemistry 44, 9417-29.
Hitosugi, T. et al. (2009) Sci Signal 2, ra73.

Published Papers

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