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Oct-1 AntibodyAB21674

  Catalog No. Package Size Price
AB21674-1 50ul $189.00
AB21674-2 100ul $279.00
AB21674-4 25ul $119.00

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Description
  • Product Name Oct-1 Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
  • Applications WB IF
  • Species Reactivity Hu Ms
  • Specificity The antibody detects endogenous levels of total Oct-1 protein.
  • Immunogen Type Peptide-KLH
  • Immunogen Description Peptide sequence around aa.270~274(T-P-K-R-I) derived from Human Oct-1.
  • Target Name Oct-1
  • Alternative Names 1-Oct; OTF1; oct-1B
  • Accession No. Swiss-Prot: P14859
    NCBI Protein: NP_001185712.1
  • Concentration 1.0mg/ml
  • Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage Store at -20℃ for long term preservation (recommended). Store at 4°C for short term use.
Application Details

Predicted MW: 90kd
Western blotting: 1:500~1:1000
Immunofluorescence:1:100~1:200
 

Images
  • Oct-1 Antibody - Absci

    Western blot analysis of extracts from JK, HepG2 and 293 cells using Oct-1 Antibody #AB21674.

  • Oct-1 Antibody - Absci

    Immunofluorescence staining of methanol-fixed NCI-h446 cells showing cytoplasmic staining using Oct-1 Antibody #AB21674.

Background

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR By similarity. In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.

Prefontaine G.G., Walther R., Giffin W. J. Biol. Chem. 274:26713-26719(1999)
Mahajan S.S., Wilson A.C. Mol. Cell. Biol. 20:919-928(2000)

    Please let us know if you have published research using #AB21674 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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