SPARC Antibody

Description

Product Name SPARC Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB
Species Reactivity Hu
Specificity The antibody detects endogenous level of total SPARC protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.52~56 (V-G-E-F-D) derived from Human SPARC.
Target Name SPARC
Alternative Names ON; BM-40;
Accession No. Swiss-Prot: P09486
NCBI Protein: NP_003109.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 42kd
Western blotting: 1:500~1:1000

Images

Western blot analysis of extract from T98G cells using SPARC Antibody #AB21633

Background

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

Kelm R.J. Jr., Mann K.G. Blood 75:1105-1113(1990)
Wewer U.M., Albrechtsen R., Fisher L.W. Am. J. Pathol. 132:345-355(1988)
Kuhn C., Mason R.J.Am. J. Pathol. 147:1759-1769(1995)

Published Papers

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