PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total FABP4 protein.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around aa.123~127(T-S-T-R-V)derived from Human FABP4.
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 15kd
Western blotting: 1:500~1:1000
Images
Western blot analysis of extract from 3T3, Hela cells using FABP4 Antibody #AB21604
Background
Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus
Marr E., Tardie M., Carty M., Brown Phillips T., Wang I.-K., Soeller W., Qiu X., Karam G. Acta Crystallogr. F 62:1058-1060(2006)