Vimentin Antibody

Description

Product Name Vimentin Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IF
Species Reactivity Human Mouse Rat Monkey
Specificity The antibody detects endogenous level of total Vimentin protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.452~456(G-Q-V-I-N) derived from Human Vimentin.
Target Name Vimentin
Alternative Names CTRCT30
Accession No. Swiss-Prot: P08670
NCBI Protein: NP_003371.2
SDS-PAGE MW 57kd
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 57kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extract from Hela, cos7 and JK cells using Vimentin Antibody #AB21488
Immunofluorescence staining of paraffin-embedded mesenchymal stem cells using Vimentin Antibody #AB21488.

Background

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.

Sommers C.L., Walker-Jones D., Heckford S.E., Worland P., Valverius E., Clark R., McCormick F.,
Cancer Res. 49:4258-4263(1989)
Kang S.-M., Shin M.-J., Kim J.-H., Oh J.-W. Proteomics 5:2227-2237(2005)
Ahmed B.A., Bukhari I.A., Jeffus B.C., Harney J.T., Thyparambil S., Ziu E., Fraer M., Rusch N.J. PLoS ONE 4:E4730-E4730(2009)

Published Papers

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