Zap-70(Ab-319) Antibody

Description

Product Name Zap-70(Ab-319) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IHC
Species Reactivity Human Mouse Rat
Specificity The antibody detects endogenous level of total Zap-70 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.317~321 (S-P-Y-S-D) derived from Human ZAP70.
Target Name Zap-70
Alternative Names 70 kDa zeta-associated protein; SRK; Syk-related tyrosine kinase; ZA70; ZAP-70
Accession No. Swiss-Prot: P43403
NCBI Protein: NP _001070.2
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 70kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from HL60 cells using Zap-70(Ab-319) Antibody #AB21173.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Zap-70(Ab-319) Antibody #AB21173(left) or the same antibody preincubated with blocking peptide(right).

Background

ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the zeta chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-zeta chain, which can mediate the transduction of extracellular stimuli into cellular effector functions

Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100(2): 443-448
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Tang J, et al. (1999) Proc Natl Acad Sci U S A; 96(17): 9775-9780
Zhao Q, et al. (1996) Mol Cell Biol; 16(12): 6765-6774
Williams BL, et al. (1999) EMBO J; 18(7): 1832-1844
Adjali O, et al. (2005) J Clin Invest; 115(8): 2287-2295.

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