cofilin(Ab-3) Antibody

Description

Product Name cofilin(Ab-3) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB IHC
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of total cofilin protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa. 1~5 (M-A-S-G-V) derived from Human cofilin.
Target Name cofilin
Alternative Names CFL; CFL1; COF1; Cofilin;
Accession No. Swiss-Prot: P23528
NCBI Protein: NP_005498.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 19kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Images

Western blot analysis of extracts from MDA cells using cofilin(Ab-3) Antibody #AB21164.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin(Ab-3) Antibody #AB21164(left) or the same antibody preincubated with blocking peptide(right).

Background

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Kobayashi M, et al. (2006) EMBO J 25(4): 713-26.
Wang Y, et al. (2005)Biol Chem 280(13): 12683-9.
Smith-Beckerman DM, et al. (2005) Mol Cell Proteomics: 156-68.

Published Papers

let us know

Customer Reviews and Rankings

No comment