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Histone H4R3me1 Polyclonal AntibodyABHW026

  Catalog No. Package Size Price
ABHW026 100ul $299.00

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Description
  • Product Name Histone H4R3me1 Polyclonal Antibody
  • Host Species Rabbit
  • Clonality Polyclonal
  • Purification Antibodies were produced by immunizing rabbits and were purified by antigen affinity-chromatography.
  • Applications WB IF IP
  • Species Reactivity Hu Ms Rt
  • Immunogen Type Peptide
  • Immunogen Description A synthetic peptide corresponding to the amino terminus of histone H4 in which Arg3 is mono-methylated.
  • Target Name Histone H4
  • Modification R3me1
  • Alternative Names H4; H4/n; H4F2; H4FN; FO108; HIST2H4
  • Accession No. Gene ID: 8290 Swiss Prot: # P62805
  • SDS-PAGE MW 11kDa
  • Concentration 1.0mg/ml
  • Formulation Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage Store at -20℃ or -80℃. Avoid freeze / thaw cycles.
Application Details

WB 1:500 - 1:2000

IF 1:50 - 1:200

IP 1:50 - 1:200

Images
  • Histone H4R3me1 Polyclonal Antibody - Absci

    Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using H4R3me1 antibody.

  • Histone H4R3me1 Polyclonal Antibody - Absci

    Immunofluorescence analysis of 293T cell using H4R3me1 antibody. Blue: DAPI for nuclear staining.

Background

 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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