PI3-kinase p85- alpha (Phospho-Tyr607) Antibody

Description

Product Name PI3-kinase p85- alpha (Phospho-Tyr607) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of PI3-kinase p85- alpha only when phosphorylated at Tyrosine 607.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of Tyrosine 607 (D-Q-Y(p)-S-L) derived from Human PI3-kinase p85-alpha.
Target Name PI3-kinase p85- alpha
Modification Phospho-Tyr607
Alternative Names p85, AGM7, GRB1, p85-ALPHA
Accession No. Swiss-Prot#: P27986;
NCBI Gene#: 5295;
NCBI Protein#: NP_001229395.1
SDS-PAGE MW 80kd
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C/1 year

Application Details

Predicted MW: 80kd
Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from Rat liver tissue using PI3-kinase p85- alpha (Phospho-Tyr607) Antibody #AB12057.The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from Hela cell treated with H2O2 using PI3-kinase p85- alpha (Phospho-Tyr607) Antibody #12057
Immunohistochemical analysis of paraffin-embedded human brain tissue tissue usingPI3-kinase p85- alpha (Phospho-Tyr607) Antibody #12057.
Lane 1 : N-peptide blocked MCF7 cell extract; Lane 2 : MCF7 cell extract; Lane 3 : P-peptide blocked MCF7 cell extract; Lane 4 : N-peptide blocked Mouse brain tissue; Lane 5 : Mouse brain tissue; Lane 6 : P-peptide blocked Mouse brain tissue; Lane 7 : N-peptide blocked Rat brain tissue; Lane 8 : Rat brain tissue; Lane 9 : P-peptide blocked Rat brain tissue; Lysates/proteins at 40 µg per lane. Predicted band size : 80 kDa Observed band size : 80 kDa

Background

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.

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