Histone H3 (Ab-27) Antibody

Description

Product Name Histone H3 (Ab-27) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Applications WB
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of total Histone H3 protein.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around aa.26-30(A-R-K-S-A) derived from Human Histone H3.
Target Name Histone H3
Alternative Names H3/A; H3FA
Accession No. Swiss-Prot: P68431
NCBI Gene ID: 8350
NCBI mRNA: NM_003529.2
NCBI Protein: NP_003520.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C

Application Details

Predicted MW: 17kd

Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from HT29 and C6 cells using Histone H3 (Ab-27) Antibody #AB21689.

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Choi H.S., Choi B.Y., Cho Y.-Y., Zhu F., Bode A.M., Dong Z.J. Biol. Chem. 280:13545-13553(2005)

Tan M., Luo H., Lee S., Jin F., Yang J.S., Montellier E., Buchou T., Cheng Z., Rousseaux S., Rajagopal N., Lu Z., Ye Z., Zhu Q., Wysocka J., Ye Y., Khochbin S., Ren B., Zhao Y.

Cell 146:1016-1028(2011)

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