VSV-G-Tag Mouse Monoclonal Antibody

Description

Product Name VSV-G-Tag Mouse Monoclonal Antibody
Host Species Mouse
Clonality Monoclonal
Isotype IgG1
Purification Affinity-chromatography
Applications WB IF/ICC
Species Reactivity Tag
Specificity VSV-G-tag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal VSV-G- tagged proteins.
Immunogen Type Peptide-KLH
Immunogen Description A synthetic peptide YTDIEMNRLGK coupled to KLH.
Target Name VSV-G-Tag
SDS-PAGE MW N/A
Concentration 1.0mg/ml
Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt

Application Details

WB: 1:3000~1:10000

IF/ICC: 1:200

Images

IP antibody use：5ug VSV-G Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with VSV-G-tag fusion protein 3、IP(untransfted 293+anti-VSV-G mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5、IP (transfected 293+anti-VSV-G mAb+ Protein G agarose) 6、IP (transfected 293+Protein G) 7、Recombinant protein (E.coli)
IF analysis of 293T cells transfected with a VSV-G-tagged protein, using VSV-G-Tag (8D6) Mouse mAb at a 1:2000 dilution (blue DAPI ,red anti-VSV-G)
1、untransfected 293 cell lysate 2、transfected 293 cell lysate with VSV-G-tag fusion protein 3、IP(untransfted 293+anti-VSV-G mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5、IP (transfected 293+anti-VSV-G mAb+ Protein G agarose) 6、IP (transfected 293+Protein G) 7、Recombinant protein (E.coli)