PCNA Mouse Monoclonal Antibody

Description

Product Name PCNA Mouse Monoclonal Antibody
Host Species Mouse
Clonality Monoclonal
Purification Affinity-chromatography
Applications WB IHC
Species Reactivity Hu Ms Rt
Immunogen Type Peptide-KLH
Immunogen Description Mouse monoclonal antibody is prepared by immunizing a synthetic peptide.
Target Name PCNA
Alternative Names Cyclin; DNA polymerase delta auxiliary protein; MGC8367; PCNA; Proliferating cell nuclear antigen.
SDS-PAGE MW 29KD
Concentration 1.0mg/ml
Formulation Mouse IgG1 in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C/1 year

Application Details

WB: 1:3000~1:10000

IHC: 1:200

Images

Western blot analysis of Hela (1), Rat brain (2), NIH 3T3 (3), 293T(4) with PCNA mouse mAb(12D10) diluted at 1:5000.

Background

PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

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