PTEN(Phospho-Ser380) Antibody

Description

Product Name PTEN(Phospho-Ser380) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications IHC IF
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of PTEN only when phosphorylated at serine 380.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 380(R-Y-S(p)-D-T) derived from Human PTEN.
Target Name PTEN
Modification Phospho-Ser380
Alternative Names BZS, DEC, GLM2, MHAM, TEP1
Accession No. Swiss-Prot: P60484
NCBI Protein: NP_000305.3
NCBI Gene ID: 5728
Calculated MW 54kd
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C

Application Details

Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images

Immunofluorescence staining of methanol-fixed HeLa cells using PTEN (phospho-Ser380) antibody (#AB11009, Red).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PTEN (phospho-Ser380) antibody (#AB11009).

Background

Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability.

Regerences

Li D.M., Sun H. Cancer Res. 57:2124-2129(1997) Song M.S., Salmena L., Carracedo A., Egia A., Lo-Coco F., Teruya-Feldstein J., Pandolfi P.P. Nature 455:813-817(2008) Myers M.P., Stolarov J.P., Eng C., Li J., Wang S.I., Wigler M.H., Parsons R., Tonks N.K. Proc. Natl. Acad. Sci. U.S.A. 94:9052-9057(1997)

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