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GAPDH mouse mAbAB21336

  Catalog No. Package Size Price
AB21336-1 50ul $189.00
AB21336-2 100ul $279.00

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Description
  • Product Name GAPDH mouse mAb
  • Host Species Mouse
  • Clonality Monoclonal
  • Purification Affinity-chromatography
  • Applications WB IHC IF E
  • Species Reactivity Hu Rt Ms Mk D ChHm Rb
  • Specificity GAPDH Mouse Monoclonal antibody detects endogenous levels of total GAPDH protein.
  • Immunogen Type Recombinant Protein
  • Immunogen Description Full-length GAPDH protein of human
  • Target Name GAPDH
  • Alternative Names G3PD, GAPD
  • Accession No. P04406
  • SDS-PAGE MW 34KD
  • Concentration 1.0mg/ml
  • Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt
  • Storage Store at -20°C/1 year
Application Details

 WB: 1:3000~1:10000
 IHC: 1:200 
 IF/ICC: 1:200

Images
  • GAPDH mouse mAb - Absci

    Western blot analysis of Hela (1), Rat brain (2), Rabbit Muscle(3), Sheep Muscle(4), and Mouse brain (5) with GAPDH mouse mAb.

Background
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.
    Please let us know if you have published research using #AB21336 so that we can cite your reference.
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    Protocol
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    Note
      Application:
    • WBWestern Blotting
    • IHCImmunohistochemistry
    • IFImmunofluorescence
    • ICCImmunocytochemistry
    • FCFlow Cytometry
    • IPImmunoprecipitation
    • EELISA
    • DBDot Blotting
    • ChIPChromatin Immunoprecipitation
    • GICAGold Immunochromatography Assay
    • NCNegative Control
      Species Reactivity:
    • HuHuman
    • MsMouse 
    • RtRat 
    • Dm Drosophila melanogaster
    • C Caenorhabditis elegans
    • MkMonkey
    • RbRabbit
    • B Bovine 
    • D Dog
    • PPig
    • HmHamster
    • ChHm Chinese Hamster 
    • ChkChicken  
    • ShpSheep  


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