PurificationThe antibody was obtained by affinity purified technique, which removes
essentially all goat serum proteins, except the specific antibody for
mouse IgG. Goat anti-mouse IgG was conjugated to peroxidase by
means of a method described by Wilson MB and Nakane PK.
ApplicationsE WB IHC
Species ReactivityMs
SpecificityThe antibody conjugate is specific for mouse IgG. No cross-reactivity
was observed with mouse IgA, IgM proteins by ELISA.
Immunogen DescriptionThe antiserum was developed in goat using the mouse IgG as the
immunogen.
ConjugatesHRP
Target NameGoat IgG
FormulationHRP-labeled Goat IgG in phosphate buffered saline (without Mg2+ and
Ca2+), pH 7.4, 150mM NaCl, 50% glycerol.
StorageStore at -20 ℃/1 year
Application Details
ELISA: 1:5000~1:20000
WB: 1:5000~1:10000
IHC: 1:1000~5000
Regerences
Wilson MB and Nakane PK. In Immunofluorescence and Related
Staining Techniques, Elsevier/North Holland Biomedical Press,