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His-Tag Mouse Monoclonal AntibodyABT505

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Description

Product Name His-Tag Mouse Monoclonal Antibody
Host Species Mouse
Clonality Monoclonal
Purification Affinity-chromatography
Applications WB IF/ICC
Species Reactivity Tag
Specificity His-Tag Mouse Monoclonal antibody detects endogenous levels of internal, C-terminal, or N-terminal 6His -tagged proteins.
Immunogen Type Peptide-KLH
Immunogen Description A synthetic peptide HHHHHH coupled to KLH.
Target Name His-Tag
SDS-PAGE MW N/A
Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt

Application Details

WB: 1:3000~1:10000

IF/ICC: 1:200

Images

IP antibody use：5ug His Mouse IgG1 per ml Lysate，WB 1:30001、untransfected 293 cell lysate2、transfected 293 cell lysate with His-tag fusion protein3、IP (untransfected 293+anti-His mAb+ Protein G agarose)4、IP (transfected 293+ normal Mouse IgG+Protein G agarose)5、IP (transfected 293+anti-His mAb+ Protein G agarose)6、Recombinant protein (E.coli)