PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsIF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of GAP43 only when phosphorylated at Ser41.
Immunogen TypePeptide-KLH
Immunogen DescriptionPeptide sequence around phosphorylation site of Ser41 (Q-A-S(p)-F-R) derived from Human GAP43.
Target NameGAP43
ModificationPhospho-Ser41
Alternative NamesB-50; BASP2; NEUM; PP46; axonal membrane protein GAP-43
Immunofluorescence staining of methanol-fixed Hela cells using GAP43(Phospho-Ser41) Antibody #AB11281.
Background
GAP43 encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
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Chantal Gamby, et,al. (1996) J. Biol. Chem; 271: 26698.