eIF2a(Phospho-Ser51) Antibody

Description

Product Name eIF2a(Phospho-Ser51) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC IF
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of eIF2a only when phosphorylated at serine 51.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 51 (E-L-S(p)-R-R) derived from Human eIF2a.
Target Name eIF2a
Modification Phospho-Ser51
Alternative Names Eukaryotic translation initiation factor 2 subunit alpha; EIF-2A;
Accession No. Swiss-Prot: P05198
NCBI Protein: NP_004085.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 38kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2a(Phospho-Ser51) Antibody #AB11279.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2α (Phospho-Ser51) Antibody #AB11279 (left) or the same antibody preincubated with blocking peptide #BP51279 (right).
Immunofluorescence staining of methanol-fixed Hela cells using eIF2a(Phospho-Ser51) Antibody #AB11279.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using eIF2α (Phospho-Ser51) Antibody #AB11279.

Background

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Xavier Saelens, et al. (2001) J. Biol. Chem; 276: 41620 - 41628.
Hiroyuki Kubota, et al. (2003) J. Biol. Chem ; 278: 20457 - 20460.
Shijian Chu, et al. (2006) Am J Physiol Lung Cell Mol Physiol ; 291: L983 - L992.
Eileen Connolly, et al. (2006) Mol. Cell. Biol ; 26: 3955 - 3965.

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