SpecificityRAD51 (Phospho-Y54) Antibody detects endogenous levels of RAD51 only when phosphorylated at Y54
Immunogen TypePeptide-KLH
Immunogen DescriptionA synthesized peptide derived from human RAD51 (Phospho-Tyr54)
Alternative NamesBRCA1 BRCA2 containing complex subunit 5 antibody
BRCC 5 antibody
BRCC5 antibody
DNA repair protein RAD51 homolog 1 antibody
DNA repair protein rhp51 antibody
FANCR antibody
hRAD51 antibody
HsRAD51 antibody
HsT16930 antibody
MRMV2 antibody
Rad 51 antibody
RAD51 antibody
RAD51 homolog (RecA homolog E. coli) (S. cerevisiae) antibody
RAD51 homolog A antibody
RAD51 homolog antibody
RAD51 recombinase antibody
RAD51 S. cerevisiae homolog of antibody
RAD51_HUMAN antibody
RAD51A antibody
RECA antibody
RecA like protein antibody
RecA E. coli homolog of antibody
Recombination protein A antibody
Calculated MW37
Concentration1.0mg mL
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) pH 7.4 150mM NaCl 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB dilution:1:1000
Images
Western blot analysis RAD51 (Phospho-Tyr54) using HeLa whole cell lysates
Product Description
DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Cells sense and repair DSBs via two distinct but partly overlapping signaling pathways, nonhomologous end joining (NHEJ) and homologous recombination (HR). Research studies have shown that defects in both pathways are associated with human disease, including cancer (reviewed in 1). ? ? DSBs that arise during S or G2 phase are repaired via homologous recombination (HR), using the replicated sister chromatid as a repair template. Rad51 recombinase, a eukaryotic homologue of E. coli RecA, polymerizes and forms a filament along single-stranded DNA, mediating HR with the help of auxiliary proteins, including Rad54 and BRCA2 (reviewed in 2,3). BRCA2 binds Rad51 and targets it to single-stranded DNA, allowing it to displace replication protein A (RPA) (4). Five Rad51 paralogs exist in vertebrates (XRCC2, XRCC3, Rad51B, Rad51C, and Rad51D) and they all appear to be required for efficient HR (5). ? ? Researchers have found that mutations in the Rad51 gene may be related to breast cancer risk (6). Some studies have implicated Rad51 as a potential marker for pancreatic cancer (7).