SpecificityPhospho-WASP(Y291) Antibody detects endogenous levels of WASP only when phosphorylated at Y291
Immunogen TypePeptide-KLH
Immunogen DescriptionA synthesized peptide derived from human WASP(Phospho-Tyr291)
Alternative NamesEczema thrombocytopenia antibody
IMD2 antibody
SCNX antibody
THC antibody
THC1 antibody
Thrombocytopenia 1 (X linked) antibody
U42471 antibody
Was antibody
WASp antibody
WASP_HUMAN antibody
Wiskott Aldrich syndrome (eczema thrombocytopenia) antibody
Wiskott Aldrich syndrome antibody
Wiskott Aldrich syndrome protein antibody
Wiskott-Aldrich syndrome protein antibody
Accession No.Swiss-Prot#:P42768 NCBI Gene ID7454
Calculated MW60
Concentration1.0mg mL
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) pH 7.4 150mM NaCl 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB dilution:1:1000
Images
Western blot analysis WASP(Phospho-Tyr291) using Heat shock treated 293 whole cell lysates
Product Description
Wiskott-Aldrich syndrome proteins (WASPs) mediate actin dynamics by activating the Arp2,3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate. Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP but lack the WASP,N-WASP autoinhibition domains and are indirectly activated by Rac (reviewed in 1). Both WASP and WAVE functions appear to be essential, as knockout of either N-WASP or Scar-2 in mice results in cardiac and neuronal defects and embryonic lethality (2,3). Loss of WASP results in immune system defects and fewer immune cells (4). WAVE-2 (WASF2) is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in brain (5). WAVE-3 may act as a tumor suppressor in neuroblastoma, a childhood disease of the sympathetic nervous system (6). Increased expression of WAVE-3 is seen in breast cancer, and studies in breast adenocarcinoma cells indicate that WAVE-3 regulates breast cancer progression, invasion and metastasis through the p38 mitogen-activated protein kinase (MAPK) pathway (7,8).