SpecificityPhospho-GRB10 (S503) Antibody detects endogenous levels of GRB10 only when phosphorylated at S503
Immunogen TypePeptide-KLH
Immunogen DescriptionA synthesized peptide derived from human GRB10 (Phospho-Ser503)
Alternative Namesgrb 10 antibody
GRB IR antibody
grb-10 antibody
GRB10 adapter protein antibody
GRB10 adaptor protein antibody
GRB10 antibody
GRB10_HUMAN antibody
GRBIR antibody
Growth factor receptor bound protein 10 antibody
Growth factor receptor-bound protein 10 antibody
Insulin receptor binding protein antibody
Insulin receptor binding protein GRB IR antibody
Insulin receptor-binding protein Grb-IR antibody
IRBP antibody
KIAA0207 antibody
Maternally expressed gene 1 antibody
MEG1 antibody
RSS antibody
Accession No.Swiss-Prot#:Q13322 NCBI Gene ID2887
Calculated MW76
Concentration1.0mg mL
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) pH 7.4 150mM NaCl 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB dilution:1:1000
Images
Western blot analysis GRB10 (Phospho-Ser503) using EGF treated HeLa whole cell lysates
Product Description
Recently GRB10 was shown to be a direct substrate of mTORC1 (5). It is phosphorylated by mTORC1 at Ser150, Ser428, and Ser476 upon insulin stimulation (5).The GRB7 family of adaptor proteins consist of GRB7, GRB10 and GRB14, which all contain an amino-terminal proline-rich SH3 binding domain, followed by PH, PBS, and SH2 domains. Each member of the family has several splice variants (1). It has been reported that GRB10 interacts with many receptor tyrosine kinases (RTKs) as well as downstream signal molecules including Raf, Akt, and Nedd4 (1,2). Although it was originally thought that GRB10 is exclusively phosphorylated at serine residues (3), Src kinase family members have been shown to phosphorylate GRB10 at Tyr67 (4).