SpecificityPhospho-Afadin (Ser1795) Antibody detects endogenous levels of Afadin only when phosphorylated at Ser1795
Immunogen TypePeptide-KLH
Immunogen DescriptionA synthesized peptide derived from human Afadin (Phospho-Ser1795)
Alternative NamesAF6 antibody
AFAD_HUMAN antibody
Afadin antibody
ALL1-fused gene from chromosome 6 protein antibody
Mllt4 antibody
Protein Af-6 antibody
Protein AF6 antibody
Accession No.Swiss-Prot#:P55196 NCBI Gene ID4301
Calculated MW205
Concentration1.0mg mL
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) pH 7.4 150mM NaCl 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB dilution:1:1000
Images
Western blot analysis Afadin (Phospho-Ser1795) using HeLa whole cell lysates
Product Description
In multicellular organisms, intercellular junctions play essential roles in tissue integrity and maintenance of cell polarity. Tight junctions (TJs) form a continuous barrier to fluids across the epithelium and endothelium (reviewed in 1). Adherens junctions (AJs) are dynamic structures that form cell-cell contacts linking cells into a continuous sheet (reviewed in 2). The actin filament-binding protein, Afadin, binds to nectin forming a connection to the action cytoskeleton (3). AJs are formed when nectin assembles cadherin at the cell-cell adhesion site and these junctions are then involved in the formation and maintenance of TJs (4, 5). Afadin has two splice variants: l-afadin, which is ubiquitously expressed, and s-afadin, which is expressed predominantly in neural tissue. s-Afadin is a shorter form lacking one of the three proline-rich regions found in l-afadin as well the carboxyl-terminal F-actin binding region (6). Human s-afadin is identical to AF-6, the ALL-1 fusion partner involved in acute myeloid leukemias (7). Recent work has also shown that afadin is involved in controlling the directionality of cell movement when it is localized at the leading edge of moving cells (8, 9).