SpecificityPhospho-LIN28A (Ser120) Antibody detects endogenous levels of LIN28A only when phosphorylated at Ser120
Immunogen TypePeptide-KLH
Immunogen DescriptionA synthesized peptide derived from human LIN28A (Phospho-Ser120)
Alternative NamesProtein lin-28 homolog A
Lin-28A
Zinc finger CCHC domain-containing protein 1
LIN28A
CSDD1 LIN28 ZCCHC1
Accession No.Swiss-Prot#:Q9H9Z2 NCBI Gene ID79727
Calculated MW26
Concentration1.0mg mL
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+) pH 7.4 150mM NaCl 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB dilution:1:1000
Images
Western blot analysis LIN28A (Phospho-Ser120) using EGF treated MCF7 whole cell lysates
Product Description
LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7).
Yes
